YEp364 vector (V010177)

MSDS

Basic Vector Information

- - Vector Name:
  - YEp364
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 8404 bp
- - Type:
  - Yeast Plasmids
- - Replication origin:
  - ori
- - Source/Author:
  - Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
- - Copy Number:
  - High copy number
- - Promoter:
  - LEU2

YEp364 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YEp364 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       YEp364.        8404 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. For other reading frames, use YEp363 or YEp365.
ACCESSION   .
VERSION     .
KEYWORDS    YEp364.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8404)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             43..3090
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3686..4777)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4778..5182)
                     /label=LEU2 promoter
     rep_origin      complement(5183..6165)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6447..6551
                     /label=AmpR promoter
     CDS             6552..7409
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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