LOCUS       YEp364.        8404 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a LEU2 marker for construction of lacZ 
            fusions. For other reading frames, use YEp363 or YEp365.
ACCESSION   .
VERSION     .
KEYWORDS    YEp364
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 8404)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             43..3090
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3686..4777)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(4778..5182)
                     /label=LEU2 promoter
     rep_origin      complement(5183..6165)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        6447..6551
                     /label=AmpR promoter
     CDS             6552..7409
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttgctc ccgccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
      301 ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt
      361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
      421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
      481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
      541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac
      601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
      661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
      721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
      781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
      841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
      901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
      961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
     2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
     2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
     2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
     3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc
     3061 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
     3121 gtatttcgcg taaggaaatc cattatgtac tatttcgcct gatgcggtat tttctcctta
     3181 cgcatctgtg cggtatttca caccgcatag atccggtacg tcgttaaggc cgtttctgac
     3241 agagtaaaat tcttgaggga actttcacca ttatggaaaa tggttcaaga aggtattgac
     3301 ttaaactcca tcaaatggtc aggtcatttg agtgtttttt atttgttgta tttttttttt
     3361 tttagagaaa atcctccaat atcaaattag ggattcgtag tttcatgatt ttctgttata
     3421 cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt gcaattctct
     3481 ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt tagctatcct
     3541 cctttttctc cttcttgata aatgtatgta gattgcgttt gtctacccta tgaacatatt
     3601 ccattttgta atttcgtgtc gtttctatta tgaatttcat ttataaagtt tatgtacaaa
     3661 tatcataaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat
     3721 caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca
     3781 aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt
     3841 tcaacatcat tgcagcagac aagatagtgg cgatagggtc aaccttattc ttggcaaatc
     3901 cgggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag
     3961 aggccaagga cgcagatggc aacaaaccca aggaacctgg ataacgtaag gcttcatcgg
     4021 agatgatatc accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct
     4081 taactaggat catggcggca gaatcaatca attgatgttg aaccttcaat gtagggaatt
     4141 cgttcttgat ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag
     4201 ctttatccaa ggtccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca
     4261 ttcttgtgat tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat
     4321 caccatcgtc ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa
     4381 cgaagtcagt acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg
     4441 atgcaaagtt acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta
     4501 aaccttgttc aggtctaaca ctaccggtac cccattttgg accacccaca gcacctaaca
     4561 aaacggcatc aaccttcttg gaggcttcca gtgcctcatc tggaagtggg acacctgtag
     4621 catcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa
     4681 catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt
     4741 cacctggcaa aacgacgatc ttcttaggaa caaacattag aatggtatat ccttgaaata
     4801 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     4861 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     4921 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcctctca
     4981 cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga
     5041 aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt
     5101 ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg
     5161 atacctgagt attcccacag ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     5221 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     5281 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     5341 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     5401 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     5461 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     5521 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     5581 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     5641 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     5701 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     5761 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     5821 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     5881 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     5941 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     6001 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     6061 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     6121 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagtatgg tgcactctca
     6181 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6241 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6301 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     6361 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8401 cccg
//
