LOCUS YEp364. 8404 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast episomal vector with a LEU2 marker for construction of lacZ fusions. For other reading frames, use YEp363 or YEp365. ACCESSION . VERSION . KEYWORDS YEp364. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8404) AUTHORS Myers AM, Tzagoloff A, Kinney DM, Lusty CJ. TITLE Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. JOURNAL Gene 1986;45:299-310. PUBMED 3026915 REFERENCE 2 (bases 1 to 8404) TITLE Direct Submission REFERENCE 3 (bases 1 to 8404) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1986"; volume: "45"; pages: "299-310" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..8404 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..36 /label=MCS /note="MCS" /note="multiple cloning site" CDS 43..3090 /label=lacZ /note="beta-galactosidase" CDS complement(3686..4777) /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" promoter complement(4778..5182) /label=LEU2 promoter rep_origin complement(5183..6165) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 6447..6551 /label=AmpR promoter CDS 6552..7409 /label=AmpR /note="beta-lactamase" rep_origin 7583..8171 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 gaattcccgg ggatccgtcg acctgcagcc aagcttgctc ccgccgtcgt tttacaacgt 61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 301 ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt 361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac 601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc 3061 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc 3121 gtatttcgcg taaggaaatc cattatgtac tatttcgcct gatgcggtat tttctcctta 3181 cgcatctgtg cggtatttca caccgcatag atccggtacg tcgttaaggc cgtttctgac 3241 agagtaaaat tcttgaggga actttcacca ttatggaaaa tggttcaaga aggtattgac 3301 ttaaactcca tcaaatggtc aggtcatttg agtgtttttt atttgttgta tttttttttt 3361 tttagagaaa atcctccaat atcaaattag ggattcgtag tttcatgatt ttctgttata 3421 cctaactttt tgtgtggtgc cctcctcctt gtcaatatta atgttaaagt gcaattctct 3481 ttccttatca cgttgagcca ttagtatcaa tttgcttacc tgtattcctt tagctatcct 3541 cctttttctc cttcttgata aatgtatgta gattgcgttt gtctacccta tgaacatatt 3601 ccattttgta atttcgtgtc gtttctatta tgaatttcat ttataaagtt tatgtacaaa 3661 tatcataaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat 3721 caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca 3781 aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt 3841 tcaacatcat tgcagcagac aagatagtgg cgatagggtc aaccttattc ttggcaaatc 3901 cgggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag 3961 aggccaagga cgcagatggc aacaaaccca aggaacctgg ataacgtaag gcttcatcgg 4021 agatgatatc accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct 4081 taactaggat catggcggca gaatcaatca attgatgttg aaccttcaat gtagggaatt 4141 cgttcttgat ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag 4201 ctttatccaa ggtccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca 4261 ttcttgtgat tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat 4321 caccatcgtc ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa 4381 cgaagtcagt acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg 4441 atgcaaagtt acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta 4501 aaccttgttc aggtctaaca ctaccggtac cccattttgg accacccaca gcacctaaca 4561 aaacggcatc aaccttcttg gaggcttcca gtgcctcatc tggaagtggg acacctgtag 4621 catcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa 4681 catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt 4741 cacctggcaa aacgacgatc ttcttaggaa caaacattag aatggtatat ccttgaaata 4801 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa 4861 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat 4921 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcctctca 4981 cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgtcagg cgacctctga 5041 aattaacaaa aaatttccag tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt 5101 ctcgttatgt tgaggaaaaa aataatggtt gctaagagat tcgaactctt gcatcttacg 5161 atacctgagt attcccacag ttaacgaagc atctgtgctt cattttgtag aacaaaaatg 5221 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 5281 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa 5341 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa 5401 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc 5461 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt 5521 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa 5581 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac 5641 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc 5701 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg 5761 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata 5821 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct 5881 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc 5941 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc 6001 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat 6061 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag 6121 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagtatgg tgcactctca 6181 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 6241 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 6301 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 6361 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 6421 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 6481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 6541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 6601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 6661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 6721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 6781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 6841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 6901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 6961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 7021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 7081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 7141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 7201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 7261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 7321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 7381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 7441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 7501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag 7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 8341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 8401 cccg //