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YIp5 vector

Basic Vector Information

Basic Vector Information
Vector Name YIp5 Antibiotic Resistance Ampicillin
Length 5541 bp Type Yeast Plasmids
Copy Number High copy number

YIp5 vector Vector Map

YIp5 vector Vector Map

YIp5 vector Sequence

LOCUS       YIp5        5541 bp    DNA     circular
DEFINITION  Yeast integrating plasmid with a URA3 marker.
ACCESSION   L09157
KEYWORDS    YIp5.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        13..41
                     /vntifkey="30"
                     /label=Promoter_P_1
                     /note="tet promoter E. coli promoter for tetracycline efflux protein  gene"
     CDS             89..1279
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /vntifkey="4"
                     /label=tet
                     /note="TcR confers resistance to tetracycline"
     promoter        1445..1660
                     /gene="S. cerevisiae URA3"
                     /vntifkey="30"
                     /label=S.\cerevisiae\URA3
                     /note="URA3 promoter"
     CDS             1661..2464
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required
                     for uracil biosynthesis"
                     /vntifkey="4"
                     /label=S.\cerevisiae\URA3
                     /note="URA3 yeast auxotrophic marker, counterselectable with  5-fluoroorotic acid (5-FOA)"
     CDS             3098..3289
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /vntifkey="4"
                     /label=rop
                     /note="rop"
     rep_origin      complement(3717..4305)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=Rep_Origin_1
                     /note="ori high-copy-number colE1/pMB1/pBR322/pUC origin of  replication"
     CDS             complement(4476..5336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=bla
                     /note="AmpR confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(5337..5441)
                     /gene="bla"
                     /vntifkey="30"
                     /label=bla
                     /note="AmpR promoter"

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