LOCUS       40924_49742        5541 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast integrating plasmid with a URA3 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5541)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..41
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             89..1276
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        1440..1660
                     /label=URA3 promoter
     CDS             1661..2461
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             3098..3286
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    3391..3531
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3717..4305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4479..5336)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5337..5441)
                     /label=AmpR promoter
ORIGIN
        1 gaattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
       61 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
      121 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
      181 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
      241 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
      301 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
      361 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
      421 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
      481 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
      541 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
      601 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
      661 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
      721 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
      781 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
      841 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
      901 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
      961 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     1021 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     1081 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     1141 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     1201 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     1261 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     1321 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     1381 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatccc cccccccctt
     1441 tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt
     1501 ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt
     1561 ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca
     1621 actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa
     1681 ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga
     1741 aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt
     1801 agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga
     1861 tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt
     1921 actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc
     1981 gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc
     2041 aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct
     2101 tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg
     2161 tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga
     2221 catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga
     2281 tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg tctctacagg
     2341 atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga
     2401 gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa
     2461 ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat
     2521 ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata atgacgaaaa
     2581 aaaaaaaatt ggaaagaaaa gggggggggg gcagcgttgg gtcctggcca cgggtgcgca
     2641 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     2701 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     2761 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     2821 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     2881 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     2941 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     3001 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     3061 ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     3121 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     3181 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     3241 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     3301 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     3361 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     3421 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     3481 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     3541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4141 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     4801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     5101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5461 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5521 acgaggccct ttcgtcttca a
//
