-
Product Name
Recombinant DT3C (Diphtheria toxin & spg 3C domain) protein
- Documents
-
Description
DT3C is a recombinant fusion protein composed of diphtheria toxin (DT) lacking its receptor-binding domain, fused to the C1, C2, and C3 IgG-binding domains of Streptococcal protein G. It acts as a versatile tool for evaluating antibody internalization, functioning like an antibody-drug conjugate (ADC) in vitro. When bound to a monoclonal antibody (mAb), DT3C is internalized into target cells, where its catalytic domain induces cytotoxicity, enabling rapid screening of internalizing antibodies for therapeutic development.
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNAs encoding 33-417aa (P00588) & 291-497aa (P19909) were fused with N-terminal 6His tag
-
Purity
>95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Validations
Recombinant DT3C (Diphtheria toxin & spg 3C domain) protein
Lane 1: DT3C, Lane 2: BSA
ADC endocytosis by DT3C
Incubate the antibody with DT3C (4 μg/mL) at 37°C for 30 min. Test the ADCs at the following concentrations: 50, 10, and 2 μg/mL. Culture the treated cells in an incubator for 2 days
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"