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Product Name
Recombinant QTGAL protein (His tag)
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Description
Recombinant Queuosine-tRNA galactosyltransferase (QTGAL) fragment (residues 1-346) from Homo sapiens, designed for structural and biochemical studies. This fragment represents a stable and soluble unit of the enzyme, facilitating investigations into its role in tRNA modification and translational regulation.
Queuosine-tRNA galactosyltransferase (QTGAL), encoded by the B3GNTL1 gene, is a glycosyltransferase that catalyzes the galactosylation of cytoplasmic tRNA(Tyr) at the queuosine-modified position 34. This modification forms galactosyl-queuosine(34), which is essential for regulating protein translation by slowing elongation at cognate codons and suppressing stop codon readthrough. The enzyme is localized in the cytoplasm, underscoring its direct involvement in cytoplasmic tRNA processing and translational control mechanisms.
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Protein name
Queuosine-tRNA galactosyltransferase
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Uniprot ID
Q67FW5
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Gene Name
B3GNTL1; B3GNT8; QTGAL
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-346 aa (Q67FW5) were fused with C-terminal 6His tag.
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Sequence
MQAHVSIILP VHNAEPWLDE CLRSVLQQDF EGTMELSVFN DASKDKSGAI IEKWRVKLED SGVHVIIGGH DSPSPRGVGY AKNQAVAQSS GSYLCFLDSD DVMMPQRVRL QHEAAVQHPS SIIGCRVRRD PPNSTERYTR WINQLTPEQL LTQVFTSNGP TVIMPTWFCS RAWFSHVGPF NEGGQGVPED LLFFYEHLRK GGGVIRVDQS LLLYRHHPQA ATHCVLETTI WTHRVRFLEE QALPRWAAFT IWNAGKQGRR LYRSLTAGSQ RKVVAFCDVD ENKIRKGFYC HEDSQERPKP RIPILHFRAA RPPFVICVKL DLTGGAFEDN LRSLHLQEGQ DFLHFSLEHH HHHH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 40.45 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 42 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant QTGAL protein (His tag) SDS-PAGE
M: Marker; Lane 1: QTGAL; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"