PA2-GNU7 peptide

PA2-GNU7 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SQRKLAAKLTSKGGGRLLRPLLQLLKQKLR-OH

H-Ser-Gln-Arg-Lys-Leu-Ala-Ala-Lys-Leu-Thr-Ser-Lys-Gly-Gly-Gly-Arg-Leu-Leu-Arg-Pro-Leu-Leu-Gln-Leu-Leu-Lys-Gln-Lys-Leu-Arg-OH

30

95.2%

C₁₅₀H₂₇₆N₅₀O₃₇

3372.09

Synthetic

Powder

PA2(SQRKLAAKLTSK) is a targeting peptide that binds specifically to OprF porin on P. aeruginosa was identified by phage display peptide library screening. GNU7(RLLRPLLQLLKQKLR) is a de novo generated short a-helical AMP, displayed broad-spectrum activities against bacteria and fungi, including against drug-resistant strains under physiological salt and serum conditions, without cytotoxicity to human cells. Hybrid peptide PA2-GNU7 exhibited potent antimicrobial activity against P. aeruginosa without causing host toxicity. Confocal laser scanning microscopy analysis and time-kill experiments demonstrated that PA2-GNU7 exhibited a high degree of specificity for P. aeruginosa, and rapidly and selectively killed P. aeruginosa cells in mixed cultures. In addition, in vivo treatment efficacy of PA2-GNU7 was significantly greater than that of conventional antibiotics in a mouse model of MDR P. aeruginosa infection.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Kim H, Jang JH, Kim SC, Cho JH. Development of a novel hybrid antimicrobial peptide for targeted killing of Pseudomonas aeruginosa. Eur J Med Chem. 2020

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.