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Product Name
Recombinant NEXN protein (His tag)
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Description
Recombinant Human Nexilin (NEXN) Fragment (aa 251-675) is a high-purity protein designed for studying the Ig-like domain's role in actin cytoskeleton regulation and sarcomere integrity. This fragment offers a focused tool for investigating cell migration and structural maintenance mechanisms.
Nexilin, encoded by the NEXN gene, is an F-actin-binding protein critical for regulating cell migration through its association with the actin cytoskeleton. It plays an essential role in maintaining Z line and sarcomere integrity, contributing to cellular structure and motility. The protein localizes to the cytoplasm, cytoskeleton, adherens junctions, and specifically to the Z line of sarcomeres, with presence at cell-matrix adherens junctions but not at cell-cell junctions. This recombinant fragment spans residues 251-675, encompassing the functional Ig-like domain (aa 582-670), enabling targeted studies of domain-specific functions in cytoskeletal dynamics and cellular organization.
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Protein name
Nexilin
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Uniprot ID
Q0ZGT2
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Gene Name
NEXN
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 251-675 aa (Q0ZGT2) were fused with N-terminal 6His tag.
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Sequence
MSKIKHHHHH HGGSFEELER QRQENRKKQA EEEARKRLEE EKRAFEEARR QMVNEDEENQ DTAKIFKGYR PGKLKLSFEE MERQRREDEK RKAEEEARRR IEEEKKAFAE ARRNMVVDDD SPEMYKTISQ EFLTPGKLEI NFEELLKQKM EEEKRRTEEE RKHKLEMEKQ EFEQLRQEMG EEEEENETFG LSREYEELIK LKRSGSIQAK NLKSKFEKIG QLSEKEIQKK IEEERARRRA IDLEIKEREA ENFHEEDDVD VRPARKSEAP FTHKVNMKAR FEQMAKAREE EEQRRIEEQK LLRMQFEQRE IDAALQKKRE EEEEEEGSIM NGSTAEDEEQ TRSGAPWFKK PLKNTSVVDS EPVRFTVKVT GEPKPEITWW FEGEILQDGE DYQYIERGET YCLYLPETFP EDGGEYMCKA VNNKGSAAST CILTIESKN
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 52.52 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 70 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"