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Product Name
Recombinant Human KRAS (C51S) Protein
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Description
This product is a soluble recombinant human KRAS4b (C51S mutant) protein (amino acid 1-169) with an N-terminal 6×His tag. The protein is expressed and purified from Escherichia coli with excellent solubility and high purity. The C51S site-specific mutation is introduced based on wild-type human KRAS4b sequence, retaining the core protein structure. This mutant protein is specially applied for KRAS functional verification, mutation mechanism study and related tumor signaling pathway research.
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Protein name
GTPase KRas
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Uniprot ID
P01116
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Gene Name
C-K-RAS; C-K-RAS; c-Ki-ras; c-Ki-ras2; CFC2; K-Ras; K-Ras 2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; OES; RALD; RALD2; RASK2
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Source/Expression Host
E.coli
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Expression Plasmid/cDNA
DNA encoding 1‑169 aa (P01116‑2, C51S) was fused with a C-terminal 6‑His tag.
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Sequence
MNHKVHHHHH HENLYFQSMT EYKLVVVGAG GVGKSALTIQ LIQNHFVDEY DPTIEDSYRK QVVIDGETSL LDILDTAGQE EYSAMRDQYM RTGEGFLCVF AINNTKSFED IHHYREQIKR VKDSEDVPMV LVGNKCDLPS RTVDTKQAQD LARSYGIPFI ETSAKTRQGV DDAFYTLVRE IRKHKEK
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 21.53 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 23.7 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant Human KRAS (C51S) Protein SDS-PAGE
M: Marker; Lane 1: KRAS (C51S); Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"