Human IL-33 recombinant protein

Cat.#: 501288

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Product Information

  • Product Name
    Human IL-33 recombinant protein
  • Documents
  • Description
    Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
  • Protein name
    Interleukin-33
  • Protein short names
    DKFZP586H0523; IL-33; C9ORF26; 9230117N10RIK; DVS27; IL33; NF-HEV; RP11-575C20.2; NFEHEV; IL1F11
  • Uniprot ID
    O95760
  • Gene Name
    IL33; C9orf26; IL1F11; NFHEV
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    A DNA sequence encoding the C-terminal segment of human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.
  • Purity
    > 98 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
    2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells costimulated with antiCD3. The ED50 for this effect is typically 0.15-0.6 ng/mL.
  • Validations

    Human IL33 / Interleukin-33 / NF-HEV Protein SDS-PAGE

    Human IL33 / Interleukin-33 / NF-HEV Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"