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Product Name
Recombinant DOT1L protein (His tag)
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Description
Recombinant Human DOT1L (1-344) is a high-purity protein fragment encompassing the DOT1 domain of Histone-lysine N-methyltransferase DOT1L. This product is engineered for studying H3 lysine-79 methylation and chromatin regulation, offering researchers a targeted tool for domain-specific functional assays.
Histone-lysine N-methyltransferase DOT1L (UniProt Q8TEK3) is a human enzyme that specifically methylates 'Lys-79' of histone H3, preferentially acting on nucleosomes over free histones. It also demonstrates DNA-binding capability, contributing to its role in chromatin modification and epigenetic regulation. DOT1L localizes to the nucleus, where it functions as a key mediator of histone methylation, influencing gene expression and cellular processes through its histone H3-K79 methyltransferase activity.
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Protein name
Histone-lysine N-methyltransferase, H3 lysine-79 specific
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Uniprot ID
Q8TEK3
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Gene Name
DOT1L; KIAA1814; KMT4
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Source/Expression Host
E. coli
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Expression Plasmid
DNA encoding 1-344 aa (Q8TEK3) were fused with C-terminal 6His tag.
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Sequence
MGEKLELRLK SPVGAEPAVY PWPLPVYDKH HDAAHEIIET IRWVCEEIPD LKLAMENYVL IDYDTKSFES MQRLCDKYNR AIDSIHQLWK GTTQPMKLNT RPSTGLLRHI LQQVYNHSVT DPEKLNNYEP FSPEVYGETS FDLVAQMIDE IKMTDDDLFV DLGSGVGQVV LQVAAATNCK HHYGVEKADI PAKYAETMDR EFRKWMKWYG KKHAEYTLER GDFLSEEWRE RIANTSVIFV NNFAFGPEVD HQLKERFANM KEGGRIVSSK PFAPLNFRIN SRNLSDIGTI MRVVELSPLK GSVSWTGKPV SYYLHTIDRT ILENYFSSLK NPKLREEQEA ARRRLEHHHH HH
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 40.90 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 44 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant DOT1L protein (His tag) SDS-PAGE
M: Marker; Lane 1: DOT1L; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"