Recombinant DEN2D protein (His tag)

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• COA (Batch NP456521)
• Material Safety Data Sheets (MSDS)

Recombinant DENN domain-containing protein 2D (DENND2D) fragment (amino acids 52-471) from Homo sapiens, encompassing the complete uDENN, cDENN, and dDENN functional domains. This high-purity fragment serves as an essential tool for investigating the guanine nucleotide exchange factor (GEF) activity and Rab protein regulation mechanisms in cellular processes.

The full-length DENN domain-containing protein 2D, encoded by the DENND2D gene, functions as a guanine nucleotide exchange factor (GEF) that activates RAB9A and RAB9B by promoting GDP-to-GTP exchange, thereby converting inactive GDP-bound Rab proteins into their active GTP-bound forms. This activity is crucial for intracellular trafficking and membrane dynamics. The protein is localized primarily in the cytoplasm, as supported by experimental evidence. The provided fragment (52-471) strategically includes all key functional domains (uDENN, cDENN, dDENN), enabling focused studies on domain-specific roles in Rab protein activation and related cell

MNHKVHFFEY LLVVSLKKKR SEDDYEPIIT YQFPKRENLL RGQQEEEERL LKAIPLFCFP DGNEWASLTE YPRETFSFVL
TNVDGSRKIG YCRRLLPAGP GPRLPKVYCI ISCIGCFGLF SKILDEVEKR HQISMAVIYP FMQGLREAAF PAPGKTVTLK
SFIPDSGTEF ISLTRPLDSH LEHVDFSSLL HCLSFEQILQ IFASAVLERK IIFLAEGLST LSQCIHAAAA LLYPFSWAHT
YIPVVPESLL ATVCCPTPFM VGVQMRFQQE VMDSPMEEVL LVNLCEGTFL MSVGDEKDIL PPKLQDDILD SLGQGINELK
TAEQINEHVS GPFVQFFVKI VGHYASYIKR EANGQGHFQE RSFCKALTSK TNRRFVKKFV KTQLFSLFIQ EAEKSKNPPA
GYFQQKILEY EEQKKQKKPR EKTVKLEHHH HHH