CLE45 peptide

CLE45 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RRVRRGSDPIHN-OH

H-Arg-Arg-Val-Arg-Arg-Gly-Ser-Asp-Pro-Ile-His-Asn-OH

12

95.1%

C₅₉H₁₀₃N₂₇O₁₇

1462.62

Synthetic

Powder

The Arabidopsis receptor kinase BAM3 is a genuine receptor of the CLE45 peptide ligand and likely acts independent of SERK co-receptors. In general, CLE peptides suppress root growth by preventing protophloem differentiation, which requires local activity of the receptor pseudokinase CRN. CRN activity promotes CLE45 sensitivity of developing protophloem by post-translational stabilization of BAM3.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hazak O, Brandt B, Cattaneo P, Santiago J, Rodriguez-Villalon A, Hothorn M, Hardtke CS. Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature. EMBO Rep. 2017 Aug;18(8):1367-1381. doi: 10.15252/embr.201643535. Epub 2017 Jun 12. PMID: 28607033; PMCID: PMC5538625.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.