Recombinant CATG protein (His tag)

• DataSheet
• COA (Batch NP456612)
• Material Safety Data Sheets (MSDS)

Recombinant Human Cathepsin G (CG) Fragment (residues 21-244) is a high-purity, functional protein designed for research on serine protease activity and immune regulation. This 224-amino acid fragment includes the complete Peptidase S1 domain, making it an ideal tool for studying proteolytic functions, antibacterial mechanisms, and cell signaling pathways in a controlled experimental setting.

Cathepsin G (EC 3.4.21.20) is a serine protease derived from Homo sapiens, encoded by the CTSG gene (UniProt ID: P08311). It exhibits trypsin- and chymotrypsin-like specificity, with a preference for Phe, Tyr, Trp, and Leu residues at the P1 position. Beyond its proteolytic roles, it possesses intrinsic antibacterial activity against both Gram-negative (e.g., Neisseria gonorrhoeae, Pseudomonas aeruginosa) and Gram-positive (e.g., Listeria monocytogenes, Staphylococcus aureus) bacteria, independent of enzymatic function. Cathepsin G is involved in platelet activation via the F2RL3/PAR4 receptor, modulates immune responses by binding to and stimulating B cells, T cells, and natural killer cells, and processes cytokines like IL36G and IL33 to enhance inflammatory signaling. It also cleaves substrates such as complement C3, vimentin, coagulation factors F8 and F10, and mucin PRG4/lubricin, impacting coagulation, tissue integrity, and infection control. Subcellularly, it localizes to the cell membrane (as a peripheral protein), cytoplasmic granules, lysosomes, cytosol, and nucleus, and is secreted by activated neutrophils into environments like synovial fluid, highlighting its dynamic roles in immunity, apoptosis, and pathogen defense.

MSKIKHHHHH HGGSIIGGRE SRPHSRPYMA YLQIQSPAGQ SRCGGFLVRE DFVLTAAHCW GSNINVTLGA HNIQRRENTQ
QHITARRAIR HPQYNQRTIQ NDIMLLQLSR RVRRNRNVNP VALPRAQEGL RPGTLCTVAG WGRVSMRRGT DTLREVQLRV
QRDRQCLRIF GSYDPRRQIC VGDRRERKAA FKGDSGGPLL CNNVAHGIVS YGKSSGVPPE VFTRVSSFLP WIRTTMRS