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Recombinant 2A5E protein (His tag)

Cat.#: 542741

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Product Information

  • Product Name
    Recombinant 2A5E protein (His tag)
  • Documents
  • Description

    Recombinant human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform (PP2A B subunit isoform B56-epsilon), featuring a stable 423-amino acid fragment (residues 45-467) optimized for structural and biochemical studies. This high-quality protein enables researchers to investigate PP2A regulatory mechanisms and substrate specificity in controlled experimental settings.

    The full-length Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform serves as a critical regulatory component of the PP2A holoenzyme. This B regulatory subunit modulates substrate selectivity and catalytic activity of the phosphatase complex, while potentially directing its localization to specific subcellular compartments. Primarily located in the cytoplasm, this protein plays a fundamental role in regulating various cellular signaling pathways through its influence on PP2A's dephosphorylation activities. The recombinant fragment provided represents a computationally optimized region that maintains structural integrity and solubility, making it ideal for mechanistic studies of PP2A regulation and function.

  • Protein name
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
  • Uniprot ID
    Q16537
  • Gene Name
    PPP2R5E
  • Source/Expression Host
    E. coli
  • Expression Plasmid
    DNA encoding 45-467 aa (Q16537) were fused with C-terminal 6His tag.
  • Sequence 
    MNHKVLTPLP LLKDVPSSEQ PELFLKKLQQ CCVIFDFMDT LSDLKMKEYK RSTLNELVDY ITISRGCLTE QTYPEVVRMV
SCNIFRTLPP SDSNEFDPEE DEPTLEASWP HLQLVYEFFI RFLESQEFQP SIAKKYIDQK FVLQLLELFD SEDPRERDYL
KTVLHRIYGK FLGLRAFIRK QINNIFLRFV YETEHFNGVA ELLEILGSII NGFALPLKAE HKQFLVKVLI PLHTVRSLSL
FHAQLAYCIV QFLEKDPSLT EPVIRGLMKF WPKTCSQKEV MFLGELEEIL DVIEPSQFVK IQEPLFKQIA KCVSSPHFQV
AERALYYWNN EYIMSLIEEN SNVILPIMFS SLYRISKEHW NPAIVALVYN VLKAFMEMNS TMFDELTATY KSDRQREKKK
EKEREELWKK LEDLELKRGL RRDGIIPTLE HHHHHH
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 51.52 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 55 kDa band.
  • Purity
    >90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.
  • Validations

    Recombinant 2A5E protein (His tag) SDS-PAGE

    Recombinant 2A5E protein (His tag) SDS-PAGE

    M: Marker; Lane 1: 2A5E; Lane 2: BSA

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"