Basic Vector Information
- Vector Name:
- psiLentGene Puromycin
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4173 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Virdugirene J.
- Promoter:
- SP6
psiLentGene Puromycin vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
psiLentGene Puromycin vector Sequence
LOCUS 40924_40412 4173 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector psiLentGene Puromycin, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4173) AUTHORS Virdugirene J. TITLE siLentGene U6 Hairpin Cloning Systems Technical Manual #TM247 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4173) AUTHORS Virdugirene J. TITLE Direct Submission JOURNAL Submitted (18-DEC-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA REFERENCE 3 (bases 1 to 4173) TITLE Direct Submission REFERENCE 4 (bases 1 to 4173) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-DEC-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4173 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 33..42 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" regulatory 37..46 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" promoter complement(140..158) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(176..192) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(200..216) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(224..254) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(269..290) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 465..822 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 851..1447 /label=PuroR /note="puromycin N-acetyltransferase" polyA_signal 1495..1543 /label=poly(A) signal /note="synthetic polyadenylation signal" rep_origin complement(1736..2324) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2498..3355) /label=AmpR /note="beta-lactamase" promoter complement(3356..3460) /label=AmpR promoter rep_origin complement(3538..3993) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 4134..4150 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 4157..4173 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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