Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | psiLentGene Hygromycin | Antibiotic Resistance | Ampicillin |
| Length | 4601 bp | Type | Cloning vector |
| Source | Virdugirene J. | ||
psiLentGene Hygromycin vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
psiLentGene Hygromycin vector Sequence
LOCUS Exported 4601 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector psiLentGene Hygromycin, complete sequence.
ACCESSION AY508734
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4601)
AUTHORS Virdugirene J.
TITLE siLentGene U6 Hairpin Cloning Systems Technical Manual #TM247
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4601)
AUTHORS Virdugirene J.
TITLE Direct Submission
JOURNAL Submitted (18-DEC-2003) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA
REFERENCE 3 (bases 1 to 4601)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4601
/organism="Cloning vector psiLentGene Hygromycin"
/mol_type="other DNA"
/db_xref="taxon:268224"
regulatory 33..42
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 37..46
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
promoter complement(140..158)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(176..192)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 200..216
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(224..254)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 269..290
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 465..822
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 673..808
/label=SV40 ori
/note="SV40 origin of replication"
CDS 863..1888
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
polyA_signal 1923..1971
/note="synthetic polyadenylation signal"
rep_origin complement(2164..2752)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2923..3783)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3784..3888)
/gene="bla"
/label=AmpR promoter
rep_origin complement(3966..4421)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(4398..180)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
RTDRPSQQLRSLNGEWTRPVAAH"
primer_bind 4562..4578
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 4585..2
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
This page is informational only.