Basic Vector Information
- Vector Name:
- psiCHECK(TM)-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3560 bp
- Type:
- RNA interference vector
- Replication origin:
- ori
- Source/Author:
- Almond BD, Vidugiriene J, Kobs G.
psiCHECK(TM)-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
psiCHECK(TM)-1 vector Sequence
LOCUS 40924_40307 3560 bp DNA circular SYN 18-DEC-2018
DEFINITION RNA interference vector psiCHECK(TM)-1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3560)
AUTHORS Almond BD, Vidugiriene J, Kobs G.
TITLE psiCHECK(TM) Vectors Technical Bulletin, TB329
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3560)
AUTHORS Almond BD, Vidugiriene J, Kobs G.
TITLE Direct Submission
JOURNAL Submitted (29-JAN-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE 3 (bases 1 to 3560)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3560)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-JAN-2004) Scientific Communications, Promega Corporation, 2800
Woods Hollow Rd., Madison, WI 53711-5399, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3560
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 62..419
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
intron 489..621
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 666..684
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 694..1626
/codon_start=1
/label=hRluc
/note="Renilla luciferase"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
misc_feature 1636..1680
/label=multiple cloning site
/note="multiple cloning site"
polyA_signal 1688..1736
/label=poly(A) signal
/note="synthetic polyadenylation signal"
promoter 1769..1873
/label=AmpR promoter
CDS 1874..2731
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2905..3493
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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