pSH476 vector (V003234)

LOCUS       40924_40072        3352 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSH476, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Hwang S-K., Okita TW.
  TITLE     Protein expression vector pSH476 (N-terminal His-tag)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Hwang S-K., Okita TW.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-APR-2008) Institute of Biological Chemistry, 
            Washington State University, Pullman, WA 99163, USA
REFERENCE   3  (bases 1 to 3352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-APR-2008) Institute of Biological Chemistry, Washington State 
            University, Pullman, WA 99163, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             166..876
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     terminator      955..1049
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    1202..1342
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1340..1440)
                     /direction=LEFT
                     /label=Ori
                     /note="Ori"
     rep_origin      complement(1528..2116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2290..3147)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3148..3252)
                     /label=AmpR promoter