pSG595 vector (V003267)

LOCUS       V003267                 9593 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003267
VERSION     V003267
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Goldfless SJ, Wagner JC, Niles JC.
  TITLE     Versatile control of Plasmodium falciparum gene expression with an
            inducible protein-RNA interaction
  JOURNAL   Nat Commun 5, 5329 (2014)
   PUBMED   25370483
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Goldfless SJ, Niles JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2014) Biological Engineering, MIT, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 9593)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9593)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            5, 5329 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-AUG-2014) Biological Engineering, MIT, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..428
                     /note="native upstream sequence from P. falciparum CRT
                     gene"
                     /regulatory_class="promoter"
     misc_feature    435..503
                     /label="mutated TetR aptamer"
                     /note="mutated TetR aptamer"
     CDS             504..1775
                     /gene="CG10"
                     /label="Chloroquine resistance transporter"
                     /note="Chloroquine resistance transporter from Plasmodium
                     falciparum (isolate 7G8). Accession#: W7FI62"
     regulatory      1787..2490
                     /label="derived from P. berghei CRT gene"
                     /note="derived from P. berghei CRT gene"
                     /regulatory_class="terminator"
     regulatory      2510..3499
                     /note="promoter and 5'UTR from P. falciparum calmodulin
                     gene"
                     /regulatory_class="promoter"
     CDS             3512..4072
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     CDS             4088..4141
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             4142..4759
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4772..4825
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4826..5377
                     /label="hGLuc"
                     /note="secreted Gaussia luciferase"
     regulatory      5435..6006
                     /label="derived from P. falciparum HRPII gene"
                     /note="derived from P. falciparum HRPII gene"
                     /regulatory_class="terminator"
     regulatory      6013..7062
                     /label="derived from P. falciparum CRT gene"
                     /note="derived from P. falciparum CRT gene"
                     /regulatory_class="terminator"
     promoter        7425..7529
                     /label="AmpR promoter"
     CDS             7530..8387
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8561..9149
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9437..9458
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9473..9503
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9511..9527
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9535..9551
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9569..9587
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"