pSCrhaB2 vector (V003405)

LOCUS       40924_39023        7518 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector pSCrhaB2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Cardona ST, Valvano MA.
  TITLE     An expression vector containing a rhamnose-inducible promoter 
            provides tightly regulated gene expression in Burkholderia 
            cenocepacia
  JOURNAL   Plasmid 54 (3), 219-228 (2005)
  PUBMED    15925406
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Cardona ST, Valvano MA.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7518)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7518)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2005"; volume: "54"; issue: "3"; pages: "219-228"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-2005) Microbiology "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(167..400)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(727..755)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(789..1634)
                     /label=rhaR
                     /note="transcriptional activator of rhaR and rhaS"
     CDS             complement(1711..2544)
                     /label=rhaS
                     /note="positive regulator of the rhaB promoter"
     promoter        2690..2808
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     regulatory      2821..2824
                     /regulatory_class="ribosome_binding_site"
     misc_feature    2828..2882
                     /label=multi-cloning site
                     /note="multi-cloning site"
     terminator      3085..3171
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3263..3290
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        complement(4145..4247)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      5640..6409
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6410..7069
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"