pScaf-8064.4 vector (V003429)

LOCUS       40924_38923       10819 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pScaf-8064.4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology, 
            University of California, San Francisco, 600 16th Street, Genentech 
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 10819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of 
            California, San Francisco, 600 16th Street, Genentech Hall S-474, 
            San Francisco, CA 94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    425..8107
                     /label=p8064.4 insert
                     /note="p8064.4 insert"
     rep_origin      8108..8437
                     /note="M13term; M13 origin modified with 3-base mutation to
                     eliminate initation capabilities, resulting in M13 
                     terminator"
     misc_feature    8388..8392
                     /label=term-alpha
                     /note="term-alpha"
     misc_feature    8406..8410
                     /label=term-beta
                     /note="term-beta"
     misc_feature    8415..8420
                     /label=term-gamma
                     /note="term-gamma"
     misc_feature    8424..8429
                     /note="term-delta-T3; TGGAAC mutated to TGGTTT"
     primer_bind     complement(8453..8469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8477..8493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8501..8531)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8546..8567)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8855..9443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9617..10474)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10475..10579)
                     /label=AmpR promoter