Basic Vector Information
pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost.
- Vector Name:
- pSBFLPS
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5769 bp
- Type:
- Cloning vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Bowden SD, Palani NP, Libourel IG.
- Copy Number:
- Low Copy
- Promoter:
- araBAD
- Growth Temperature:
- 30℃
pSBFLPS vector Map
pSBFLPS vector Sequence
LOCUS 40924_38808 5769 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pSBFLPS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5769)
AUTHORS Bowden SD, Palani NP, Libourel IG.
TITLE Stringent control of FLP recombinase in Escherichia coli
JOURNAL J. Microbiol. Methods 133, 52-54 (2016)
PUBMED 28024983
REFERENCE 2 (bases 1 to 5769)
AUTHORS Bowden SD, Palani NP, Libourel IG.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2016) Biotechnology Institute, University of
Minnesota, 1500 Gortner Avenue, Saint Paul, MN 55108, USA
REFERENCE 3 (bases 1 to 5769)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5769)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J.
Microbiol. Methods 133, 52-54 (2016)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-DEC-2016) Biotechnology Institute, University of Minnesota, 1500
Gortner Avenue, Saint Paul, MN 55108, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5769
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 27..131
/label=AmpR promoter
CDS 132..989
/label=AmpR
/note="beta-lactamase"
terminator 996..1035
/label=fd terminator
/note="central terminator from bacteriophage fd (Otsuka and
Kunisawa, 1982)"
oriT 1182..1290
/label=oriT
/note="incP origin of transfer"
rep_origin 1841..2063
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 2111..3058
/label=Rep101
/note="RepA protein needed for replication with the pSC101
origin"
CDS complement(3256..4524)
/label=FLP
/note="site-specific recombinase"
RBS 4535..4543
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
regulatory complement(4536..4541)
/regulatory_class="ribosome_binding_site"
promoter complement(4570..4854)
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 4881..5756
/label=araC
/note="L-arabinose regulatory protein"
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