pSAP2-g-multi vector (V003512)

LOCUS       40924_38463        7095 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plasmid vector pSAP2-g-multi DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Miichi F.
  TITLE     Construction of plasmid vector pSAP2-g-multi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Miichi F, Yoshida H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2017) Contact:Fumika Miichi Saga University, 
            Department of Biomolecular Sciences; 5-1-1 Nabeshima, Saga, Saga 
            849-8501, Japan
REFERENCE   3  (bases 1 to 7095)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7095)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JUN-2017) Contact:Fumika Miichi Saga University, Department of 
            Biomolecular Sciences; 5-1-1 Nabeshima, Saga, Saga 849-8501, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           659..1249
                     /gene="EhActin 5' region"
     gene            659..1249
                     /gene="EhActin 5' region"
                     /label=EhActin 5' region
     CDS             1250..2041
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     5'UTR           2051..3056
                     /gene="EhActin 3' region"
     gene            2051..3056
                     /gene="EhActin 3' region"
                     /label=EhActin 3' region
     rep_origin      3077..4363
                     /label=Autonomous replication sequence
                     /note="Autonomous replication sequence"
     5'UTR           4370..4843
                     /gene="EhAP-a 5' region"
     gene            4370..4843
                     /gene="EhAP-a 5' region"
                     /label=EhAP-a 5' region
     promoter        complement(4910..4928)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4949..4965)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4973..4989)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4997..5027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5042..5063)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5351..5939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6113..6970)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6971..7075)
                     /label=AmpR promoter