pRV-9 vector (V003539)

LOCUS       V003539                10427 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003539
VERSION     V003539
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Nishizawa M, Fu SL, Kataoka K, Vogt PK.
  TITLE     Artificial oncoproteins: modified versions of the yeast bZip protein
            GCN4 induce cellular transformation
  JOURNAL   Oncogene 22 (39), 7931-7941 (2003)
   PUBMED   12970741
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   Nishizawa M, Fu S-L., Kataoka K, Vogt PK.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2002) Dept. of Molecular and Experimental
            Medicine, The Scripps Research Institute, 10550 North Torrey Pines
            Road, La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 10427)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene";
            date: "2003"; volume: "22"; issue: "39"; pages: "7931-7941"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-FEB-2002) Dept. of Molecular and Experimental Medicine, The
            Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla,
            CA 92037, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        411..676
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    745..762
                     /note="tRNA-Trp primer binding site for reverse
                     transcription"
     CDS             2752..3123
                     /gene="gag"
                     /label="Proteinase p15"
                     /note="Proteinase p15 from Avian myeloblastosis associated
                     virus. Accession#: P26315"
     CDS             3144..5831
                     /codon_start=1
                     /gene="pol"
                     /product="reverse trascriptase"
                     /label="pol"
                     /note="RNA-dependent DNA polymerase; derived from Bryan
                     high-titer strain"
                     /protein_id="AAL91562.1"
                     /translation="TVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLVEKELQLG
                     HIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSALPRGWPLM
                     VLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTICQLVVGQ
                     VLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPDKIQREPG
                     VQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMGPFYEQLR
                     GSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIGVLGQGLS
                     THPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPACFREDLPL
                     PEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDASSSTHKG
                     VVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVAKMLLKMG
                     QEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATFQAYPLRE
                     AKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLGPLQIWQT
                     DFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRPKAIKTDN
                     GSCFTSRSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGDGFMKRIP
                     TSKQGELLAKAMYALNHFERGENTKTPIQKHWRPTVLTEGPPVKIRIETGEWEKGWNVL
                     VWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWIPWEDEQE
                     GLQGETASNKQERPGEDTLAANES"
     gene            3144..5831
                     /gene="pol"
                     /label="pol"
     CDS             6784..7521
                     /gene="env"
                     /label="Envelope glycoprotein gp95"
                     /note="Envelope glycoprotein gp95 from Rous sarcoma virus
                     subgroup A (strain Schmidt-Ruppin). Accession#: P0DTM5"
     misc_feature    7674..7722
                     /label="polylinker sequence"
                     /note="polylinker sequence"
     repeat_region   7835..8167
     primer_bind     complement(8206..8222)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8230..8246
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8254..8284)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8299..8320)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8608..9196)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9370..10227)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10228..10332)
                     /label="AmpR promoter"