pRT106 vector (V003542)

LOCUS       V003542                 8221 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003542
VERSION     V003542
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Thomas R, Chege G, Shephard E, Bourn W, Maclean J, Jaffray A, Tanzer
            F, Steyn L, Berkower C, Jacobs W Jr., Rybicki E, Williamson A-L.
  TITLE     Construction and stability analysis of different recombinant BCG
            expressing HIV subtype C Pr55 Gag that induce an immune response in
            baboons which can be boosted by HIV-1 subtype C Pr55 Gag virus-like
            particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Thomas R, Chege G, Shephard E, Bourn W, Maclean J, Jaffray A, Tanzer
            F, Steyn L, Berkower C, Jacobs W Jr., Rybicki E, Williamson A-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Cape Town, Western Cape 7925, South Africa
REFERENCE   3  (bases 1 to 8221)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8221)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JAN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Cape Town, Western Cape 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             5698..6330
                     /gene="tmk"
                     /label="Thymidylate kinase"
                     /note="Thymidylate kinase from Mycobacterium tuberculosis
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6556..8034
                     /codon_start=1
                     /product="Gag"
                     /label="Gag"
                     /note="HIV-1 Gag from subtype C isolate DU422 from South
                     Africa"
                     /protein_id="AAX11265.1"
                     /translation="MGARASILRGEKLDTWEKIRLRPGGKKHYMLKHIVWASRELERFA
                     LNPGLLETSEGCKQIIKQLQPALQTGTEELKSLYDTVATLYCVHEKIEVRDTKEALDKI
                     EEEQNKCQQKTQQAKAADGKVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVIEEKAFSP
                     EVIPMFTALSEGATPQDLNTMLNTVGGHQAAMQMLKDTINEEAAEWDRLHPVHAGPIAP
                     GQMREPRGSDIAGTTSTLQEQIAWMTSNPPIPVGDIYKRWIILGLNKIVRMYSPVSILD
                     IRQGPKEPFRDYVDRFFKTLRAEQATQEVKNWMTDTLLVQNANPDCKTILRALGPGATL
                     EEMMTACQGVGGPGHKARVLAEAMSQTNSGNIMMQRSNFKGPRRIVKCFNCGKEGHIAR
                     NCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQNRPEPTAPPAES
                     FRLEETTPAPKQEPIEREPLTSLKSLFGSDPLSQ"
     terminator      8139..8185
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"