pTn7oluxK3 vector (V002588)

Basic Vector Information

      • Vector Name:
      • pTn7oluxK3
      • Length:
      • 9823 bp
      • Type:
      • Promoter capture vector
      • Source/Author:
      • Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.

pTn7oluxK3 vector Vector Map

pTn7oluxK39823 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600oriTR6K gamma oriTn7LattB1PompA regionLuxCLuxDLuxALuxBLuxEattB2FRTNeoR/KanRFRTrrnB T1 terminatorlambda t0 terminatorKS primerTn7R

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTn7oluxK3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_43688        9823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter capture vector pTn7oluxK3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Tn5/7 lux : A versatile vector for the location, capture, and 
            utilization of Gram negative promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Bruckbauer ST, Kvitko BH, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2012) Microbiology Immunology and Pathology, 
            Colorado State University, IDRC at Foothills Campus, Campus Delivery
            0922, Fort Collins, CO 80523-0922, USA
REFERENCE   3  (bases 1 to 9823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2012) Microbiology Immunology and Pathology, Colorado State 
            University, IDRC at Foothills Campus, Campus Delivery 0922, Fort 
            Collins, CO 80523-0922, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            365..473
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      496..880
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  1096..1261
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     protein_bind    1292..1316
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1347..1554
                     /label=PompA region
                     /note="PompA region"
                     /regulatory_class="promoter"
     CDS             1581..3020
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     CDS             3035..3955
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             4007..5086
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             5104..6084
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             6266..7375
                     /label=LuxE
                     /note="LuxE"
     protein_bind    complement(7413..7437)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     7493..7540
                     /label=FRT
                     /note="FRT"
     protein_bind    complement(7493..7540)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7926..8717
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(8819..8866)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(8912..8998)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(9101..9195)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(9223..9239)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    9244..9442
                     /label=Tn7R
                     /note="Tn7R"

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