pTL85 vector (V002610)

LOCUS       40924_43588        8197 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTL85, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Construction and characterization of centromeric, episomal and 
            GFP-containing vectors for Saccharomyces cerevisiae prototrophic 
            strains
  JOURNAL   J. Biotechnol. 143 (4), 247-254 (2009)
  PUBMED    19683551
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2009) Department of Genetics, Biology of 
            Microorganisms, Anthropology, Evolution, University of Parma, Viale 
            Usberti 11/A, Parma 43100, Italy
REFERENCE   3  (bases 1 to 8197)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8197)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2009"; volume: "143"; issue: "4"; pages: 
            "247-254"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2009) Department of Genetics, Biology of Microorganisms, 
            Anthropology, Evolution, University of Parma, Viale Usberti 11/A, 
            Parma 43100, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(249..496)
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(509..1222)
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     1225..1241
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1275..1291)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1292..1641)
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     complement(1686..1702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(2139..3485)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     gene            3639..4995
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     promoter        complement(5788..6185)
                     /label=LEU2 promoter
     promoter        6291..6395
                     /label=AmpR promoter
     CDS             6396..7253
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7427..8015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"