ptkLUC+ vector (V002616)

MSDS

Basic Vector Information

- - Vector Name:
  - ptkLUC+
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 4711 bp
- - Type:
  - Eukaryotic luciferase expression vector
- - Replication origin:
  - ori
- - Source/Author:
  - Altschmied J, Duschl J.
- - Promoter:
  - Mini-TK

ptkLUC+ vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

ptkLUC+ vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_43563        4711 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Eukaryotic luciferase expression vector ptkLUC+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Altschmied J, Duschl J.
  TITLE     Set of optimized luciferase reporter gene plasmids compatible with 
            widely used CAT vectors
  JOURNAL   BioTechniques 23 (3), 436-438 (1997)
  PUBMED    9298214
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Altschmied J, Duschl J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-1997) Physiological Chemistry I/Biocenter, 
            University of Wuerzburg, Am Hubland, Wuerzburg 97074, Germany
REFERENCE   3  (bases 1 to 4711)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1997"; volume: "23"; issue: "3"; pages: 
            "436-438"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-1997) Physiological Chemistry I/Biocenter, University of 
            Wuerzburg, Am Hubland, Wuerzburg 97074, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=polylinker
                     /note="polylinker"
     promoter        112..174
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     CDS             238..1887
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1931..2052)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2146..2162
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(2184..2200)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2208..2224)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2232..2262)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2277..2298)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2586..3174)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3348..4205)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4206..4310)
                     /label=AmpR promoter
     polyA_signal    4337..4471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4574..4708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"

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