pTK1940 vector (V002624)

LOCUS       V002624                 9431 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002624
VERSION     V002624
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Kafri T.
  TITLE     Superior lentiviral vectors designed for BSL-0 environment abolish
            vector mobilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Kafri T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Gene Therapy Center, University of North
            Carolina at Chapel Hill, 104 Manning Dr., Chapel Hill, NC 27599, US
REFERENCE   3  (bases 1 to 9431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Gene Therapy Center, University of North Carolina at
            Chapel Hill, 104 Manning Dr., Chapel Hill, NC 27599, US"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..817
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             832..1012
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1674..1907
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2092..2136
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2285..2326
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2395..2512
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        complement(2530..2547)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(2565..2686)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(2908..3496)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(3543..4259)
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        complement(4304..4507)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(4508..4811)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   5122..5413
                     /label="3' LTR"
                     /note="3' LTR"
     LTR             5232..5412
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5444..5668
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5714..6142
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6156..6486
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        6534..6581
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             6600..6971
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7104..7237
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7274..7290)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7298..7314)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7322..7352)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7367..7388)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7676..8264)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8438..9295)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9296..9400)
                     /label="AmpR promoter"