Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pTip-NH2 | Length | 8169 bp |
| Type | Expression vector | Source | Nakashima N, Tamura T. |
pTip-NH2 vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTip-NH2 vector Sequence
LOCUS Exported 8169 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pTip-NH2 DNA, complete sequence.
ACCESSION AB094067
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8169)
AUTHORS Nakashima N, Tamura T.
TITLE A novel system for expressing recombinant proteins over a wide
temperature range from 4 to 35 degrees C
JOURNAL Biotechnol. Bioeng. 86 (2), 136-148 (2004)
PUBMED 15052633
REFERENCE 2 (bases 1 to 8169)
AUTHORS Nakashima N, Tamura T.
TITLE Direct Submission
JOURNAL Submitted (17-OCT-2002) Nobutaka Nakashima, National Institute of
Advanced Industrial Science and Technology; Tsukisamu-Higashi
2-17-2-1, Sapporo, Toyohira-ku 062-8517, Japan
(E-mail:n-nakashima@aist.go.jp, Tel:81-11-857-8937)
REFERENCE 3 (bases 1 to 8169)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8169
/organism="Expression vector pTip-NH2"
/mol_type="other DNA"
/db_xref="taxon:211425"
CDS 170..979
/codon_start=1
/gene="tsr"
/product="thiostrepton resistance protein"
/label=tsr
/protein_id="BAD11220.1"
/translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
gene 170..979
/gene="tsr"
/label=tsr
regulatory 1145..1324
/regulatory_class="promoter"
/gene="Tuf1"
gene 1145..1324
/gene="Tuf1"
/label=Tuf1
CDS 1325..2515
/codon_start=1
/gene="Tetr"
/product="tetracycline resistance protein"
/label=Tetr
/protein_id="BAD11221.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
gene 1325..2515
/gene="Tetr"
/label=Tetr
regulatory 2728..2903
/regulatory_class="promoter"
/gene="ThcA"
gene 2728..2903
/gene="ThcA"
/label=ThcA
CDS 2904..3665
/codon_start=1
/gene="TipA"
/label=TipA
/protein_id="BAD11222.1"
/translation="MSYSVGQVAGFAGVTVRTLHHYDDIGLLVPSERSHAGHRRYSDAD
LDRLQQILFYRELGFPLDEVAALLDDPAADPRAHLRRQHELLSARIGKLQKMAAAVEQA
MEARSMGINLTPEEKFEVFGDFDPDQYEEEVRERWGNTDAYRQSKEKTASYTKEDWQRI
QDEADELTRRFVALMDAGEPADSEGAMDAAEDHRQGIARNHYDCGYEMHTCLGEMYVSD
ERFTRNIDAAKPGLAAYMRDAILANAVRHTP"
gene 2904..3665
/gene="TipA"
/label=TipA
promoter 3843..3947
/gene="bla"
/label=AmpR promoter
CDS 3948..4808
/codon_start=1
/gene="Ampr"
/product="ampicillin resistance protein"
/label=Ampr
/protein_id="BAD11223.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene 3948..4808
/gene="Ampr"
/label=Ampr
rep_origin 4809..5811
/label=ColE1
/note="ColE1"
rep_origin 4979..5567
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
regulatory complement(5818..5998)
/regulatory_class="terminator"
/gene="ThcA"
gene complement(5818..5998)
/gene="ThcA"
/label=ThcA
CDS complement(6063..6080)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
gene complement(6087..6229)
/gene="TipA"
/label=TipA
regulatory complement(6087..6105)
/regulatory_class="ribosome_binding_site"
/gene="TipA"
regulatory complement(6106..6229)
/regulatory_class="promoter"
/gene="TipA"
CDS 6768..7655
/codon_start=1
/gene="RepA"
/label=RepA
/protein_id="BAD11224.1"
/translation="MKSATGGVLSDRQWADAVLLPHRPFATNNLQRGQYRMSRDDALAM
RYVEHSPHALLGSIVIDCDHVDAAMRAFEQPSDHPAPNWVAQSPSGRAHIGWWLGPNHV
CRTDSARLTPLRYAHRIETGLKISVGGDFAYGGQLTKNPIHPDWETIYGPATPYTLRQL
ATIHTPRQMPRRPDRAVGLGRNVTMFDATRRWAYPQWWQHRNGTGRDWDHLVLQHCHAV
NTEFTTPLPFTEVRATAQSISKWIWRNFTEEQYRARQAHLGQKGGKATTLAKQEAVRNN
ARKYDEHTMREAII"
gene 6768..7655
/gene="RepA"
/label=RepA
CDS 7655..7939
/codon_start=1
/gene="RepB"
/label=RepB
/protein_id="BAD11225.1"
/translation="MGGAKNPVRRKMTAAAAAEKFGASTRTIQRLFAEPRDDYLGRAKA
RRDKAVELRKQGLKYREIAEAMELSTGIVGRLLHDARRHGEISAEDLSA"
gene 7655..7939
/gene="RepB"
/label=RepB
This page is informational only.