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pTip-NH2 vector (V002632#)

Basic Vector Information

Basic Vector Information
Vector Name pTip-NH2 Length 8169 bp
Type Expression vector Source Nakashima N, Tamura T.

pTip-NH2 vector Vector Map

tsr Tuf1 Tetr ThcA TipA AmpR promoter Ampr ColE1 ThcA 6xHis TipA RepA RepB

Plasmid Resuspension protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTip-NH2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8169 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pTip-NH2 DNA, complete sequence.
ACCESSION   AB094067
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     A novel system for expressing recombinant proteins over a wide 
            temperature range from 4 to 35 degrees C
  JOURNAL   Biotechnol. Bioeng. 86 (2), 136-148 (2004)
  PUBMED    15052633
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Nakashima N, Tamura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2002) Nobutaka Nakashima, National Institute of 
            Advanced Industrial Science and Technology; Tsukisamu-Higashi 
            2-17-2-1, Sapporo, Toyohira-ku 062-8517, Japan 
            (E-mail:n-nakashima@aist.go.jp, Tel:81-11-857-8937)
REFERENCE   3  (bases 1 to 8169)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Expression vector pTip-NH2"
                     /mol_type="other DNA"
                     /db_xref="taxon:211425"
     CDS             170..979
                     /codon_start=1
                     /gene="tsr"
                     /product="thiostrepton resistance protein"
                     /label=tsr
                     /protein_id="BAD11220.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            170..979
                     /gene="tsr"
                     /label=tsr
     regulatory      1145..1324
                     /regulatory_class="promoter"
                     /gene="Tuf1"
     gene            1145..1324
                     /gene="Tuf1"
                     /label=Tuf1
     CDS             1325..2515
                     /codon_start=1
                     /gene="Tetr"
                     /product="tetracycline resistance protein"
                     /label=Tetr
                     /protein_id="BAD11221.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            1325..2515
                     /gene="Tetr"
                     /label=Tetr
     regulatory      2728..2903
                     /regulatory_class="promoter"
                     /gene="ThcA"
     gene            2728..2903
                     /gene="ThcA"
                     /label=ThcA
     CDS             2904..3665
                     /codon_start=1
                     /gene="TipA"
                     /label=TipA
                     /protein_id="BAD11222.1"
                     /translation="MSYSVGQVAGFAGVTVRTLHHYDDIGLLVPSERSHAGHRRYSDAD
                     LDRLQQILFYRELGFPLDEVAALLDDPAADPRAHLRRQHELLSARIGKLQKMAAAVEQA
                     MEARSMGINLTPEEKFEVFGDFDPDQYEEEVRERWGNTDAYRQSKEKTASYTKEDWQRI
                     QDEADELTRRFVALMDAGEPADSEGAMDAAEDHRQGIARNHYDCGYEMHTCLGEMYVSD
                     ERFTRNIDAAKPGLAAYMRDAILANAVRHTP"
     gene            2904..3665
                     /gene="TipA"
                     /label=TipA
     promoter        3843..3947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3948..4808
                     /codon_start=1
                     /gene="Ampr"
                     /product="ampicillin resistance protein"
                     /label=Ampr
                     /protein_id="BAD11223.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3948..4808
                     /gene="Ampr"
                     /label=Ampr
     rep_origin      4809..5811
                     /label=ColE1
                     /note="ColE1"
     rep_origin      4979..5567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(5818..5998)
                     /regulatory_class="terminator"
                     /gene="ThcA"
     gene            complement(5818..5998)
                     /gene="ThcA"
                     /label=ThcA
     CDS             complement(6063..6080)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     gene            complement(6087..6229)
                     /gene="TipA"
                     /label=TipA
     regulatory      complement(6087..6105)
                     /regulatory_class="ribosome_binding_site"
                     /gene="TipA"
     regulatory      complement(6106..6229)
                     /regulatory_class="promoter"
                     /gene="TipA"
     CDS             6768..7655
                     /codon_start=1
                     /gene="RepA"
                     /label=RepA
                     /protein_id="BAD11224.1"
                     /translation="MKSATGGVLSDRQWADAVLLPHRPFATNNLQRGQYRMSRDDALAM
                     RYVEHSPHALLGSIVIDCDHVDAAMRAFEQPSDHPAPNWVAQSPSGRAHIGWWLGPNHV
                     CRTDSARLTPLRYAHRIETGLKISVGGDFAYGGQLTKNPIHPDWETIYGPATPYTLRQL
                     ATIHTPRQMPRRPDRAVGLGRNVTMFDATRRWAYPQWWQHRNGTGRDWDHLVLQHCHAV
                     NTEFTTPLPFTEVRATAQSISKWIWRNFTEEQYRARQAHLGQKGGKATTLAKQEAVRNN
                     ARKYDEHTMREAII"
     gene            6768..7655
                     /gene="RepA"
                     /label=RepA
     CDS             7655..7939
                     /codon_start=1
                     /gene="RepB"
                     /label=RepB
                     /protein_id="BAD11225.1"
                     /translation="MGGAKNPVRRKMTAAAAAEKFGASTRTIQRLFAEPRDDYLGRAKA
                     RRDKAVELRKQGLKYREIAEAMELSTGIVGRLLHDARRHGEISAEDLSA"
     gene            7655..7939
                     /gene="RepB"
                     /label=RepB

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