pTHSSe_70 vector (V002649)

LOCUS       V002649                 7391 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002649
VERSION     V002649
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Segall-Shapiro TH, Sontag ED, Voigt CA.
  TITLE     Engineered promoters enable constant gene expression at any copy
            number in bacteria
  JOURNAL   Nat. Biotechnol. (2018) In press
   PUBMED   29553576
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Segall-Shapiro TH, Sontag ED, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2018) Synthetic Biology Center, Department of
            Biological Engineering, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7391)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7391)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAR-2018) Synthetic Biology Center, Department of Biological
            Engineering, Massachusetts Institute of Technology, 77 Massachusetts
            Avenue, Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(13..102)
                     /label="ECK120029600"
                     /note="ECK120029600"
                     /regulatory_class="terminator"
     regulatory      107..175
                     /label="PT7A1w1"
                     /note="PT7A1w1"
                     /regulatory_class="promoter"
     misc_RNA        177..227
                     /label="sTRSV HHRz"
                     /note="hammerhead ribozyme from the tobacco ringspot virus
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      251..273
                     /label="RBSVioEc"
                     /note="RBSVioEc"
                     /regulatory_class="ribosome_binding_site"
     CDS             274..849
                     /codon_start=1
                     /product="VioE"
                     /label="VioE"
                     /protein_id="AVR55197.1"
                     /translation="MENREPPLLPARWSSAYVSYWSPMLPDDQLTSGYCWFDYERDICR
                     IDGLFNPWSERDTGYRLWMSEVGNAASGRTWKQKVAYGRERTALGEQLCERPLDDETGP
                     FAELFLPRDVLRRLGARHIGRRVVLGREADGWRYQRPGKGPSTLYLDAASGTPLRMVTG
                     DEASRASLRDFPNVSEAEIPDAVFAAKR"
     regulatory      850..873
                     /label="RBSVioAc"
                     /note="RBSVioAc"
                     /regulatory_class="ribosome_binding_site"
     CDS             874..2127
                     /gene="vioA"
                     /label="Flavin-dependent L-tryptophan oxidase VioA"
                     /note="Flavin-dependent L-tryptophan oxidase VioA from
                     Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 /
                     JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757).
                     Accession#: Q9S3V1"
     regulatory      2131..2154
                     /label="RBSVioBc"
                     /note="RBSVioBc"
                     /regulatory_class="ribosome_binding_site"
     CDS             2155..5148
                     /gene="vioB"
                     /label="2-imino-3-(indol-3-yl)propanoate dimerase"
                     /note="2-imino-3-(indol-3-yl)propanoate dimerase from
                     Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 /
                     JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757).
                     Accession#: Q9S3V0"
     terminator      5166..5237
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5253..5280
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    5299..5319
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      5320..5377
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      5549..6137
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6338..7129)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      complement(7324..7367)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    7370..7391
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"