Basic Vector Information
- Vector Name:
- pTha-M1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6242 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Tanaka R, Kikutani S, Mahardika A, Matsuda Y.
pTha-M1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTha-M1 vector Sequence
LOCUS 40924_43243 6242 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pTha-M1 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6242)
AUTHORS Tanaka R, Kikutani S, Mahardika A, Matsuda Y.
TITLE Localization of enzymes relating to C4 organic acid metabolisms in
the marine diatom, Thalassiosira pseudonana
JOURNAL Photosyn. Res. 121 (2-3), 251-263 (2014)
PUBMED 24414292
REFERENCE 2 (bases 1 to 6242)
AUTHORS Kikutani S, Matsuda Y.
TITLE Direct Submission
JOURNAL Submitted (30-AUG-2013) Contact:Sae Kikutani Kwansei Gakuin
University, Department of Bioscience; 2-1 Gakuen, Sanda, Hyogo
669-1337, Japan
REFERENCE 3 (bases 1 to 6242)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6242)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Photosyn.
Res. 121 (2-3), 251-263 (2014)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-AUG-2013) Contact:Sae Kikutani Kwansei Gakuin University,
Department of Bioscience; 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6242
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 670..686
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 701..1417
/label=EGFP
/note="enhanced GFP"
regulatory 1429..1931
/label=Tpfcp8
/note="Tpfcp8"
/regulatory_class="terminator"
regulatory 1958..2916
/label=Tpfcp8
/note="Tpfcp8"
/regulatory_class="promoter"
CDS 2924..3490
/label=NrsR
/note="nourseothricin acetyltransferase"
regulatory 3493..3996
/label=Tpfcp8
/note="Tpfcp8"
/regulatory_class="terminator"
primer_bind complement(3997..4013)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(4054..4072)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4093..4109)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4117..4133)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4141..4171)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4186..4207)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4495..5083)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5257..6114)
/label=AmpR
/note="beta-lactamase"
promoter complement(6115..6219)
/label=AmpR promoter
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