pTEX5501ts vector (V002746)

LOCUS       V002746                 6279 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002746
VERSION     V002746
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Nallapareddy SR, Singh KV, Murray BE.
  TITLE     Construction of Improved Temperature-Sensitive and Mobilizable
            Vectors and Their Use for Constructing Mutations in the
            Adhesin-Encoding acm Gene of Poorly Transformable Clinical
            Enterococcus faecium Strains
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 334-345 (2006)
   PUBMED   16391062
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Nallapareddy SR, Singh KV, Murray BE.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2005) Infectious Diseases, Univ. of Texas Med.
            School at Houston, 6431 Fannin St, MSB 2.112, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 6279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "1"; pages:
            "334-345"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-SEP-2005) Infectious Diseases, Univ. of Texas Med. School at
            Houston, 6431 Fannin St, MSB 2.112, Houston, TX 77030, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..61
                     /note="multiple cloning site I; MCS-I"
     CDS             195..842
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    1007..1076
                     /note="multiple cloning site II; MCS-II"
     CDS             complement(1479..1490)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1624..1833
                     /codon_start=1
                     /gene="orfB"
                     /product="OrfB"
                     /label="orfB"
                     /note="portion of pWV01 based temperature-sensitive
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20801.1"
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIVVFMITK"
     gene            1624..1833
                     /gene="orfB"
                     /label="orfB"
     CDS             1874..2035
                     /codon_start=1
                     /product="OrfC"
                     /label="OrfC"
                     /note="portion of pWV01 based temperature-sensitive
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20802.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     CDS             2102..2800
                     /codon_start=1
                     /gene="repA(ts)"
                     /product="RepA(ts)"
                     /label="repA(ts)"
                     /note="portion of pWV01 based temperature-sensitive
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20803.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDLYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            2102..2800
                     /gene="repA(ts)"
                     /label="repA(ts)"
     CDS             2797..2946
                     /codon_start=1
                     /gene="orfD"
                     /product="OrfD"
                     /label="orfD"
                     /note="portion of pWV01 based temperature-sensitive
                     gram-positive replicon derived from pTV1-OK"
                     /protein_id="ABB20804.1"
                     /translation="MTNKEKELFAENEELKKEIKDLKERIERYREMEVELSTTIDLLRG
                     GIIE"
     gene            2797..2946
                     /gene="orfD"
                     /label="orfD"
     CDS             3173..4078
                     /codon_start=1
                     /gene="aph(2'')-Id"
                     /product="APH(2'')-Id"
                     /label="aph(2'')-Id"
                     /note="aminoglycoside modifying enzyme; derived from
                     Enterococcus casseliflavus in GenBank Accession Number
                     AF016483"
                     /protein_id="ABB20805.1"
                     /translation="MRTYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEINRDFIF
                     KFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLT
                     PLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSR
                     ELKGPQMKIVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAA
                     ISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKE
                     YGYMDWYEEGLNEIRSIKIK"
     gene            3173..4078
                     /gene="aph(2'')-Id"
                     /label="aph(2'')-Id"
     oriT            4172..4838
                     /note="oriT of Tn916 derived from pHS1; facilitates
                     conjugative transfer from the transformable E. faecalis
                     strain JH2Sm::Tn916 to E. faecium"
     rep_origin      complement(5036..5624)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"