pTcR-HG-Neo-plus vector (V002767)

MSDS

Basic Vector Information

- - Vector Name:
  - pTcR-HG-Neo-plus
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3536 bp
- - Type:
  - Cloning vector
- - Replication origin:
  - ori
- - Source/Author:
  - Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira CA.

pTcR-HG-Neo-plus vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTcR-HG-Neo-plus vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_42904        3536 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTcR-HG-Neo-plus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Plasmids and molecular building blocks to assist the development and
            extension of genetic manipulation tools for Trypanosoma cruzi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Bouvier LA, de los Milagros Camara M, Canepa GE, Miranda MR, Pereira
            CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Laboratorio de Biologia Molecular de 
            Trypanosoma cruzi, Instituto de Investigaciones Medicas (IDIM) 
            Alfredo Lanari, Combatientes de Malvinas 3150, CABA, Buenos Aires 
            1427, Argentina
REFERENCE   3  (bases 1 to 3536)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3536)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Laboratorio de Biologia Molecular de Trypanosoma 
            cruzi, Instituto de Investigaciones Medicas (IDIM) Alfredo Lanari, 
            Combatientes de Malvinas 3150, CABA, Buenos Aires 1427, Argentina"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             267..1058
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(1815..2403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2577..3434)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(join(3435..3536,1..3))
                     /label=AmpR promoter

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