pTCH-PB vector (V002792)

LOCUS       V002792                 7750 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002792
VERSION     V002792
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Shao M, Kumar S, Thomson JG.
  TITLE     Precise excision of plastid DNA by the large serine recombinase Bxb1
  JOURNAL   Plant Biotechnol. J. (2013) In press
   PUBMED   24261912
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Shao M, Kumar S, Thomson JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2013) Crop Improvement and Utilization, USDA, 800
            Buchanan Street, Albany, CA 94710, USA
REFERENCE   3  (bases 1 to 7750)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7750)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Plant
            Biotechnol. J. (2013) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-SEP-2013) Crop Improvement and Utilization, USDA, 800 Buchanan
            Street, Albany, CA 94710, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..824
                     /note="plastid sequence targeting insertions in the
                     trnI-trnA intergenic region II; similar to INSD Accession
                     Number Z00044"
     rep_origin      complement(1073..1661)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1835..2692)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2693..2797)
                     /label="AmpR promoter"
     misc_feature    3021..4438
                     /note="plastid sequence targeting insertions in the
                     trnI-trnA intergenic region I; similar to INSD Accession
                     Number Z00044"
     regulatory      4470..4586
                     /label="plastid rRNA operon PEP promoter (Prrn)"
                     /note="plastid rRNA operon PEP promoter (Prrn)"
                     /regulatory_class="promoter"
     5'UTR           4591..4607
                     /note="psbA; similar to INSD Accession Number NC_001879"
     CDS             4610..5398
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     protein_bind    complement(5445..5478)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_recomb     5517..5622
                     /note="ParA resolution site; similar to INSD Accession
                     Number M59825"
     misc_recomb     5634..5752
                     /note="CinH resolution site; similar to INSD Accession
                     Number AF213017"
     misc_recomb     5792..5843
                     /note="Bxb1 attP52 recognition site; similar to INSD
                     Accession Number JQ305140"
     misc_recomb     5904..5960
                     /note="phiC31 attP57 recognition site; similar to INSD
                     Accession Number GU56446"
     regulatory      6017..6166
                     /note="rps16 3'UTR; similar to INSD Accession Number
                     NC_001879"
                     /regulatory_class="terminator"
     regulatory      6176..6278
                     /note="psbA; similar to INSD Accession Number NC_001879"
                     /regulatory_class="promoter"
     5'UTR           6279..6363
                     /note="psbA; similar to INSD Accession Number NC_001879"
     CDS             6366..7208
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from
                     Mycolicibacterium fortuitum. Accession#: Q49184"
     misc_recomb     7218..7258
                     /note="Bxb1 attB42 recognition site; similar to INSD
                     Accession Number JQ305140"
     regulatory      7289..7479
                     /note="psbA 3'UTR; similar to INSD Accession Number
                     NC_001879"
                     /regulatory_class="terminator"
     misc_feature    7525..7703
                     /note="plastid DNA targeting sequence; similar to inverted
                     repeat sequence INSD Accession Number Z00044"