pTc6HisP-NH 1.1 vector (V002805)

Basic Vector Information

      • Vector Name:
      • pTc6HisP-NH 1.1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6477 bp
      • Type:
      • Protein fusion vector
      • Source/Author:
      • Kugeratski FG, Batista M, Inoue AH, Ramos BD, Krieger MA, Marchini/ FK.

pTc6HisP-NH 1.1 vector Vector Map

pTc6HisP-NH 1.16477 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300f1 oriM13 fwdT7 promoterderived from Trypanosoma cruzi 18S ribosomal RNA gene promoter region6xHisattR1lac UV5 promoterCmRccdBattR2BleoRT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTc6HisP-NH 1.1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_42704        6477 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Protein fusion vector pTc6HisP-NH 1.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Kugeratski FG, Batista M, Inoue AH, Ramos BD, Krieger MA, Marchini/ 
            FK.
  TITLE     pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma 
            cruzi gene characterisation
  JOURNAL   Mem. Inst. Oswaldo Cruz (2015) In press
  PUBMED    26200713
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Kugeratski FG, Batista M, Inoue AH, Ramos BD, Krieger MA, Marchini 
            FK.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Mass Spectrometry Platform, Instituto Carlos
            Chagas, Professor Algacyr Munhoz Mader, 3775, Curitiba, Parana 
            81350010, Brasil
REFERENCE   3  (bases 1 to 6477)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6477)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mem. Inst. 
            Oswaldo Cruz (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2015) Mass Spectrometry Platform, Instituto Carlos Chagas, 
            Professor Algacyr Munhoz Mader, 3775, Curitiba, Parana 81350010, 
            Brasil"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..1275
                     /note="derived from Trypanosoma cruzi 18S ribosomal RNA
                     gene promoter region"
                     /regulatory_class="promoter"
     CDS             1569..1586
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    1596..1715
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1745..1775
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1829..2485
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2830..3132
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3176..3300)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3609..3980
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(4289..4307)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4328..4344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4352..4368)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4376..4406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4421..4442)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4730..5318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5492..6349)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6350..6454)
                     /label=AmpR promoter

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