pTargeT vector (V002808)

MSDS

Basic Vector Information

Vector Name:: pTargeT

Antibiotic Resistance:: Ampicillin

Length:: 5670 bp

Type:: Mammalian expression vector

Replication origin:: ori

Source/Author:: Promega Corporation.

Promoter:: CMV

pTargeT vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTargeT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_42684        5670 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pTargeT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Promega Corporation.
  JOURNAL   (in) PTARGET(TM) MAMMALIAN EXPRESSION SYSTEM TECHINCAL MANUAL. 
            Promega Corporation (2004)
REFERENCE   2  (bases 1 to 5670)
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2004) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            PTARGET(TM) MAMMALIAN EXPRESSION SYSTEM TECHINCAL MANUAL. Promega 
            Corporation (2004)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-FEB-2004) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Road, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1229..1247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1250..1323
                     /label=multiple cloning region
                     /note="multiple cloning region"
     primer_bind     1344..1367
                     /label=pTargeT(tm) sequencing primer
                     /note="pTargeT(tm) sequencing primer"
     protein_bind    complement(1397..1413)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1421..1451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1466..1487)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(1543..1664)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1798..2252
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2267..2624
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2675..3466
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3533..3581
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        3873..3977
                     /label=AmpR promoter
     CDS             3978..4835
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5009..5597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

This page is informational only.