Basic Vector Information
- Vector Name:
- pTac-Pg6MSAS-sfp
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9940 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Kim B, Binkley R, Kim HU, Lee SY.
- Promoter:
- tac
pTac-Pg6MSAS-sfp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTac-Pg6MSAS-sfp vector Sequence
LOCUS 40924_42459 9940 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pTac-Pg6MSAS-sfp, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9940)
AUTHORS Kim B, Binkley R, Kim HU, Lee SY.
TITLE Metabolic engineering of Escherichia coli for the enhanced
production of l-tyrosine
JOURNAL Biotechnol. Bioeng. (2018) In press
PUBMED 30019750
REFERENCE 2 (bases 1 to 9940)
AUTHORS Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
Korea Advanced Institute of Science and Technology, Daehak-ro 291,
Daejeon 34141, South Korea
REFERENCE 3 (bases 1 to 9940)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9940)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
Bioeng. (2018) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
34141, South Korea"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9940
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 9..5330
/note="6-methylsalicylic acid synthase from Penicillium
patulum. Accession#: P22367"
promoter 5451..5479
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 5487..5503
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 5526..6197
/gene="sfp"
/label=sfp
/note="4'-phosphopantetheinyl transferase Sfp from Bacillus
subtilis (strain 168). Accession#: P39135"
misc_feature 6201..6257
/label=MCS
/note="pUC18/19 multiple cloning site"
terminator 6460..6546
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 6638..6665
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 6685..6776
/label=AmpR promoter
primer_bind complement(7089..7105)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 7348..8160
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 8692..9237
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
regulatory 9874..9902
/label=Tac promoter
/note="Tac promoter"
/regulatory_class="promoter"
promoter 9874..9902
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 9910..9926
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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