pT7MT vector (V002845)

LOCUS       V002845                 5664 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002845
VERSION     V002845
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Marikar FM, Fang L, Jiang SH, Hua ZC.
  TITLE     pT7MT, a metallothionein 2A-tagged novel prokaryotic fusion
            expression vector
  JOURNAL   J. Microbiol. Biotechnol. 17 (5), 728-732 (2007)
   PUBMED   18051292
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Marikar FMM.T., Hua Z-C.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2006) State Key Lab of Pharmaceutical
            Biotechnology, Nanjing University, 20 HanKou Road, Nanjing, Jiangsu
            210093, P.R.China
REFERENCE   3  (bases 1 to 5664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Biotechnol."; date: "2007"; volume: "17"; issue: "5";
            pages: "728-732"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-APR-2006) State Key Lab of Pharmaceutical Biotechnology, Nanjing
            University, 20 HanKou Road, Nanjing, Jiangsu 210093, P.R.China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /label="AmpR promoter"
     CDS             599..1456
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1630..2218
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2404..2546)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2651..2839)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3614..3635)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3651..4730)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4731..4808)
                     /label="lacI promoter"
     promoter        5121..5139
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5140..5164
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5173..5238
                     /note="Omega sequence; leader sequence from tobacco mosaic
                     virus; MTV U2 RAA; tobacco mosaic virus U2"
     RBS             5254..5276
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5289..5462
                     /gene="MT2A"
                     /label="Metallothionein-2"
                     /note="Metallothionein-2 from Homo sapiens. Accession#:
                     P02795"
     CDS             5463..5474
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    5476..5526
                     /note="multiple cloning site; MCS; designed for inserting
                     fusion partners"
     terminator      5592..5639
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"