pSpiro-NptII-ESP-C-SF-TAP vector (V003069)

LOCUS       40924_41126        6781 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Episomal vector pSpiro-NptII-ESP-C-SF-TAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Jerlstrom-Hultqvist J, Einarsson E, Svard SG.
  TITLE     Stable Transfection of the Diplomonad Parasite Spironucleus 
            salmonicida
  JOURNAL   Eukaryotic Cell 11 (11), 1353-1361 (2012)
  PUBMED    22983987
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Jerlstroem-Hultqvist JSE., Einarsson EM, Svaerd SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology, 
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 6781)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2012"; volume: "11"; issue: "11"; pages: "1353-1361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala 
            University, BMC, Box 596, Uppsala 751 24, Sweden"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     3'UTR           complement(2214..2593)
                     /note="fructose bisphosphate aldolase from Spironucleus 
                     salmonicida"
     CDS             complement(2594..3385)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     5'UTR           complement(3386..3678)
                     /note="fructose bisphosphate aldolase from Spironucleus 
                     salmonicida"
     5'UTR           3738..4398
                     /note="encystation-specific protease-like protein from 
                     Spironucleus salmonicida"
     CDS             5935..5958
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             5995..6018
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             6034..6057
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     3'UTR           6064..6123
                     /note="ribosomal protein S15A from Spironucleus
                     salmonicida"
     promoter        complement(6138..6156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6166..6182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6324..6779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"