pSpiro-BSR-2xOLLAS-C vector (V003074)

LOCUS       V003074                 3894 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003074
VERSION     V003074
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Jerlstrom-Hultqvist J, Einarsson E, Svard SG.
  TITLE     Stable Transfection of the Diplomonad Parasite Spironucleus
            salmonicida
  JOURNAL   Eukaryotic Cell 11 (11), 1353-1361 (2012)
   PUBMED   22983987
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Jerlstroem-Hultqvist JSE., Einarsson EM, Svaerd SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology,
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 3894)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2012"; volume: "11"; issue: "11"; pages: "1353-1361"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala
            University, BMC, Box 596, Uppsala 751 24, Sweden"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     3'UTR           complement(2214..2423)
                     /note="ornithine carbamoyltransferase from Spironucleus
                     salmonicida"
     CDS             complement(2430..2849)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     5'UTR           complement(2847..3011)
                     /note="ornithine carbamoyltransferase from Spironucleus
                     salmonicida"
     CDS             3087..3128
                     /label="OLLAS tag"
                     /note="Escherichia coli OmpF Linker and mouse Langerin
                     fusion sequence tag (Park et al., 2008)"
     CDS             3129..3170
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin
                     fusion sequence tag (Park et al., 2008)"
                     /label="OLLAS tag"
                     /translation="SGFANELGPRLMGK"
     3'UTR           3177..3236
                     /note="ribosomal protein S15A from Spironucleus
                     salmonicida"
     promoter        complement(3251..3269)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3279..3295)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3437..3892
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"