Basic Vector Information
- Vector Name:
- FUW-tetO-hKLF4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9904 bp
- Type:
- Mammalian Expression, Lentiviral
- Replication origin:
- ori
- Copy Number:
- High Copy
- Promoter:
- EM7
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- LNCX
FUW-tetO-hKLF4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
FUW-tetO-hKLF4 vector Sequence
LOCUS FUW-tetO-hKLF4. 9904 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS FUW-tetO-hKLF4. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9904) AUTHORS Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R TITLE A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):346-53. PUBMED 18786421 REFERENCE 2 (bases 1 to 9904) TITLE Direct Submission REFERENCE 3 (bases 1 to 9904) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem Cell. 2008 Sep 11. 3(3):346-53." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..9904 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 7..386 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 387..589 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 604..784 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 831..956 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1449..1682 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1867..1911 /label=gp41 peptide /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 2060..2101 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" misc_feature 2209..2326 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2385..2655 /label=tetracycline response element /note="contains seven copies of the tetracycline operator tetO" promoter 2688..2725 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2722..2746 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2901..4337 /label=hKLF4 /note="Homo sapiens Kruppel-like factor 4 (Klf4) gene. Belongs to the relatively large family of SP1-like transcription factors and is involved in the regulation of proliferation, differentiation, apoptosis and somatic cell reprogramming." misc_feature 4365..4953 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4956..4972) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 5478..5658 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 5690..5914 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5960..6388 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6402..6731 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 6779..6826 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 6845..7216 /label=BleoR /note="antibiotic-binding protein" polyA_signal 7349..7482 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7519..7535) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(7543..7559) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7567..7597) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(7612..7633) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7750..7767) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7921..8509) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8683..9540) /label=AmpR /note="beta-lactamase" promoter complement(9541..9645) /label=AmpR promoter primer_bind complement(9720..9739) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker"
This page is informational only.