Basic Vector Information
- Vector Name:
- HDAC1 Flag
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6869 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Selection Marker:
- Neomycin (select with G418)
- Copy Number:
- High Copy
- Promoter:
- SV40
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- T7
- 3' Primer:
- BGH Reverse
HDAC1 Flag vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
HDAC1 Flag vector Sequence
LOCUS HDAC1_Flag. 6869 bp DNA circular SYN 13-MAY-2021
DEFINITION Mammalian expression of human histone deacetylase 1 with flag tag.
ACCESSION .
VERSION .
KEYWORDS HDAC1 Flag.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6869)
AUTHORS Emiliani S, Fischle W, Van Lint C, Al-Abed Y, Verdin E
TITLE Characterization of a human RPD3 ortholog, HDAC3.
JOURNAL Proc Natl Acad Sci U S A. 1998 Mar 17. 95(6):2795-800.
PUBMED 9501169
REFERENCE 2 (bases 1 to 6869)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6869)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl
Acad Sci U S A. 1998 Mar 17. 95(6):2795-800."
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6869
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 74..453
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 454..657
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 702..720
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 783..2228
/gene="HDAC1"
/label=HDAC1
/note="Histone deacetylase 1 from Homo sapiens. Accession#:
Q13547"
CDS 2241..2264
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
polyA_signal 2305..2529
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2575..3003
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3017..3346
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3413..4204
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 4381..4514
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4551..4567)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4575..4591)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4599..4629)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4644..4665)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4782..4799)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4953..5541)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5715..6572)
/label=AmpR
/note="beta-lactamase"
promoter complement(6573..6677)
/label=AmpR promoter
primer_bind complement(6752..6771)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
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