pSKPDR5-PPUS vector (V003152)

LOCUS       40924_40597        5654 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSKPDR5-PPUS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Nakamura K, Niimi M, Niimi K, Holmes AR, Yates JE, Decottignies A, 
            Monk BC, Goffeau A, Cannon RD.
  TITLE     Functional expression of Candida albicans drug efflux pump Cdr1p in 
            a Saccharomyces cerevisiae strain deficient in membrane transporters
  JOURNAL   Antimicrob. Agents Chemother. 45 (12), 3366-3374 (2001)
  PUBMED    11709310
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Lamping E, Niimi M, Cannon RD.
  TITLE     Small, synthetic, GC-rich mRNA stem-loop modules 5' proximal to the 
            AUG start-codon predictably tune gene expression in yeast
  JOURNAL   Microb. Cell Fact. 12 (1), 74 (2013)
  PUBMED    23895661
REFERENCE   3  (bases 1 to 5654)
  AUTHORS   Lamping E, Niimi M, Cannon RD.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2011) Sir John Walsh Research Institute, 
            University of Otago, 310 Great King Street, Dunedin, Otago 9054, New
            Zealand
REFERENCE   4  (bases 1 to 5654)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5654)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "2001"; volume: "45"; issue: "12"; pages: 
            "3366-3374"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Microb. 
            Cell Fact."; date: "2013"; volume: "12"; issue: "1"; pages: "74"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-AUG-2011) Sir John Walsh Research Institute, University of 
            Otago, 310 Great King Street, Dunedin, Otago 9054, New Zealand"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     667..683
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1823..1864
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      1870..2069
                     /label=3' end of Saccharomyces cerevisiae PDR5 ORF
                     /note="3' end of Saccharomyces cerevisiae PDR5 ORF"
                     /regulatory_class="terminator"
     promoter        2106..2321
                     /label=URA3 promoter
     CDS             2322..3122
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    3146..3423
                     /label=3' end of Saccharomyces cerevisiae PDR5 ORF
                     /note="3' end of Saccharomyces cerevisiae PDR5 ORF"
     promoter        complement(3466..3484)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3505..3521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3529..3545)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3553..3583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3598..3619)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3907..4495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4669..5526)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5527..5631)
                     /label=AmpR promoter