Basic Vector Information
psiSTRIKE Puromycin vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
psiSTRIKE Puromycin vector Sequence
LOCUS 40924_40487 4364 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector psiSTRIKE Puromycin, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4364)
TITLE Direct Submission
JOURNAL Submitted (09-DEC-2003) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE 2 (bases 1 to 4364)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 4364)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(09-DEC-2003) Scientific Communications, Promega Corporation, 2800
Woods Hollow Rd., Madison, WI 53711, USA"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4364
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 27..267
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
promoter complement(331..349)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(367..383)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(391..407)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(415..445)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(460..481)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 656..1013
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 1042..1638
/codon_start=1
/label=PuroR
/note="puromycin N-acetyltransferase"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 1686..1734
/label=poly(A) signal
/note="synthetic polyadenylation signal"
rep_origin complement(1927..2515)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2689..3546)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3547..3651)
/label=AmpR promoter
rep_origin complement(3729..4184)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 4298..4321
/label=pUC/M13 forward sequencing primer
/note="pUC/M13 forward sequencing primer"
primer_bind 4325..4341
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 4348..4364
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
This page is informational only.