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psiSTRIKE Hygromycin vector (V003174#)

Basic Vector Information

Basic Vector Information
Vector Name psiSTRIKE Hygromycin Antibiotic Resistance Ampicillin
Length 4792 bp Type Cloning vector

psiSTRIKE Hygromycin vector Vector Map

U6 SP6 RNA polymerase M13 rev lac operator lac promoter CAP binding site SV40 enhancer and early promoter hygromycin phosphotransferase synthetic ori b-lactamase AmpR promoter f1 ori pUC/M13 forward sequencing primer M13 fwd

psiSTRIKE Hygromycin vector Sequence

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LOCUS       Exported                4792 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector psiSTRIKE Hygromycin, complete sequence.
ACCESSION   AY497506
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4792)
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Cloning vector psiSTRIKE Hygromycin"
                     /mol_type="other DNA"
                     /db_xref="taxon:258974"
     regulatory      16..275
                     /regulatory_class="promoter"
                     /note="U6"
     promoter        27..267
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     regulatory      330..349
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase"
     promoter        complement(331..349)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(367..380)
                     /label=pUC/M13 reverse sequencing primer
                     /note="pUC/M13 reverse sequencing primer"
     protein_bind    391..407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(415..445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    460..481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    601..1019
                     /label=SV40 enhancer and early promoter
                     /note="SV40 enhancer and early promoter"
     promoter        656..1013
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      864..999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      917..982
                     /label=SV40 minimum origin of replication
                     /note="SV40 minimum origin of replication"
     CDS             1054..2079
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /label=hygromycin phosphotransferase
                     /protein_id="AAR91725.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     regulatory      2114..2162
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic"
     polyA_signal    2114..2162
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2355..2943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3114..3974)
                     /codon_start=1
                     /product="b-lactamase"
                     /label=b-lactamase
                     /note="AmpR"
                     /protein_id="AAR91726.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3975..4079)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4157..4612)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4726..4749
                     /label=pUC/M13 forward sequencing primer
                     /note="pUC/M13 forward sequencing primer"
     primer_bind     4753..4769
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4776..3
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase"
     promoter        4776..2
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"

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