Basic Vector Information
- Vector Name:
- pPAH
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3407 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Kobayashi H.
pPAH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPAH vector Sequence
LOCUS 40924_34051 3407 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pPAH DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3407)
AUTHORS Kobayashi H.
TITLE Inducible suppression of global translation by overuse of rare
codons
JOURNAL Appl. Environ. Microbiol. 81 (7), 2544-2553 (2015)
PUBMED 25636849
REFERENCE 2 (bases 1 to 3407)
AUTHORS Kobayashi H.
TITLE Direct Submission
JOURNAL Submitted (07-JAN-2015) Contact:Hideki Kobayashi Agency for
Marine-Earth Science and Technology, Institute of Biogeosciences;
2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL
:http://www.jamstec.go.jp/j/
REFERENCE 3 (bases 1 to 3407)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3407)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2015"; volume: "81"; issue: "7"; pages:
"2544-2553"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-JAN-2015) Contact:Hideki Kobayashi Agency for Marine-Earth
Science and Technology, Institute of Biogeosciences; 2-15
Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL
:http://www.jamstec.go.jp/j/"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3407
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 200..913
/codon_start=1
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNGIKV
NFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMILLE
FVTAAGITHGMDELYK"
terminator 1127..1213
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1305..1332
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1352..1443
/label=AmpR promoter
CDS 1444..2301
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
terminator 2334..2428
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
rep_origin 2516..3104
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(3268..3354)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
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