Basic Vector Information
- Vector Name:
- pOpt_mVenus_Hyg
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6864 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lauersen KJ, Mussgnug JH, Kruse O.
- Promoter:
- T3
pOpt_mVenus_Hyg vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOpt_mVenus_Hyg vector Sequence
LOCUS 40924_33857 6864 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pOpt_mVenus_Hyg, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6864) AUTHORS Lauersen KJ, Mussgnug JH, Kruse O. TITLE Efficient targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a novel, modular vector toolkit JOURNAL Unpublished REFERENCE 2 (bases 1 to 6864) AUTHORS Lauersen KJ, Mussgnug JH, Kruse O. TITLE Direct Submission JOURNAL Submitted (25-JUN-2014) Algae Biotechnology and Bioenergy, University of Bielefeld Center for Biotechnology, Universitaetsstr 27, Bielefeld, NRW 33615, Germany REFERENCE 3 (bases 1 to 6864) TITLE Direct Submission REFERENCE 4 (bases 1 to 6864) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (25-JUN-2014) Algae Biotechnology and Bioenergy, University of Bielefeld Center for Biotechnology, Universitaetsstr 27, Bielefeld, NRW 33615, Germany" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6864 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 489..505 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 515..533 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1247..1258 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" CDS 2302..2313 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" CDS 2326..2349 /label=Strep-Tag II /note="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" CDS 3299..4294 /gene="hyg" /label=hyg /note="Hygromycin-B 7''-O-kinase from Streptomyces hygroscopicus. Accession#: P09979" promoter complement(4564..4582) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4603..4619) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4627..4643) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4651..4681) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4696..4717) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5005..5593) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5767..6624) /label=AmpR /note="beta-lactamase" promoter complement(6625..6729) /label=AmpR promoter rep_origin complement(join(6756..6864,1..347)) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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