Basic Vector Information
- Vector Name:
- pOmni
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3710 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Ma Y.
pOmni vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOmni vector Sequence
LOCUS V004232 3710 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004232
VERSION V004232
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 3710)
AUTHORS Ma Y.
TITLE Development of a dual-expression vector facilitated with
selection-free PCR recombination cloning strategy
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3710)
AUTHORS Ma Y.
TITLE Direct Submission
JOURNAL Submitted (13-FEB-2017) Rongchang Campus, Southwest University, No.
2 Tiansheng Road, Beibei District, Chongqing, Chongqing 400715,
China
REFERENCE 3 (bases 1 to 3710)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3710)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-FEB-2017) Rongchang Campus, Southwest University, No. 2
Tiansheng Road, Beibei District, Chongqing, Chongqing 400715, China"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3710
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 239..618
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 619..822
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 867..885
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 905..929
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 944..966
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
misc_feature 970..976
/label="Kozak sequence"
/note="Kozak sequence"
CDS 985..1002
/label="6xHis"
/note="6xHis affinity tag"
CDS 1264..1536
/gene="E"
/label="Lysis protein E"
/note="Lysis protein E from Enterobacteria phage phiX174.
Accession#: P03639"
polyA_signal 1569..1793
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
terminator 1796..1843
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin complement(1958..2543)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2717..3574)
/label="AmpR"
/note="beta-lactamase"
promoter complement(3575..3679)
/label="AmpR promoter"
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