pOmni vector (V004232)

LOCUS       V004232                 3710 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004232
VERSION     V004232
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma Y.
  TITLE     Development of a dual-expression vector facilitated with
            selection-free PCR recombination cloning strategy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Ma Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2017) Rongchang Campus, Southwest University, No.
            2 Tiansheng Road, Beibei District, Chongqing, Chongqing 400715,
            China
REFERENCE   3  (bases 1 to 3710)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3710)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-FEB-2017) Rongchang Campus, Southwest University, No. 2
            Tiansheng Road, Beibei District, Chongqing, Chongqing 400715, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        239..618
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..822
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        867..885
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    905..929
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             944..966
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    970..976
                     /label="Kozak sequence"
                     /note="Kozak sequence"
     CDS             985..1002
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1264..1536
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"
     polyA_signal    1569..1793
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     terminator      1796..1843
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      complement(1958..2543)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2717..3574)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3575..3679)
                     /label="AmpR promoter"